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Sequencing Reaction with Big Dye v3.0

  Standard Standard
  1/16 1/8
H20 5.625 5.125
5X 1.75 1.75
BigDye 0.5 1
Primer (100 μm) 0.125 0.125
DNA (10-75ng) 2 2
Total volume 10 10

 
 

Kelly B. Miller Lab, Department of Biology, University of New Mexico, 167 Castetter Hall, MSC03 2020, Albuquerque, NM 87131-0001 USA
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